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Safranin O (C.I. 50240) is a CERTISTAIN dry dye that is used for the preparation of a staining solution for e.g. Gram stain procedures in smears and sections of human origin in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.
It is mostly utilized for the identification of cartilage, mucin, and mast cell granules. The safranin stain works by binding to acidic proteoglycans in cartilage tissues with a high affinity forming a reddish-orange complex. The binding made cartilage tissues appear red when observed under the microscope.
How does safranin affect Gram-positive cells? Safranin penetrates the cell wall, but not enough of it is retained to cause a color change...... In the Gram-positive cell walls, most of the spaces between the molecules that make up peptidoglycan are already occupied by crystal violet/iodine complexes.
BioGnost's Gram Safranin solution is used for contrast staining of bacterial species that did not retain their primary dye, i. e. Gram-negative bacteria. That enables differentiating the blue and purple-colored Gram-positive bacteria from the red-colored Gram-negative bacteria.
Oman, Bahrain, Abu Dhabi, Ajman, Al Ain, Dubai, Ras Al-Khaimah, Fujairah, Sharjah, Umm Al Quwain, Ruwais, Mina Zayed, Khalifa Port, Kizad, Jebel Ali, Port Rashid, Khor Fakkan, Sharjah Creek, Port of Hamriyah, Mussafah Port, Saqr Port, Dibba Port, Jebel Dhanna, Mina Al Hamriya, and all major UAE ports.