Skip to content
Test Kit For Water

THIOGLYCOLATE INDICATOR

Product code RXSOL-62-6047-111

Thioglycolate (thioglycolic acid) is used in water testing as a powerful reducing and complexing agent to measure both dissolved and total iron.

Description

Thioglycolate (thioglycolic acid) is used in water testing as a powerful reducing and complexing agent to measure both dissolved and total iron. It works by reducing insoluble ferric iron (Fe³⁺) to ferrous iron (Fe²⁺) and binding with it to form a highly visible purple complex.

Thioglycolic acid is used for the reduction of iron(III) to iron(II), and for pH adjustment. Thus only one reagent is required for analysis.

Application
Application
How the Thioglycolate Method Works

 

  • Reduction: Thioglycolic acid reduces oxidized ferric iron (Fe³⁺) into soluble ferrous iron (Fe²⁺).
  •  

  • Complexation: The ferrous iron reacts with the thioglycolate to form a purple or pink-colored ferrous thioglycolate complex.
  •  

  • Measurement: The intensity of the resulting color is directly proportional to the total iron concentration in the water sample
  • Typical Testing Procedure
    1. Color Comparison: After a brief waiting period (usually 3 to 5 minutes), the color of the sample is compared against a standard color chart or measured photometrically to determine the iron level in mg/L (or ppm).
    Advantages and Limitations
    • Interferences: The formulation used in many test kits is designed to minimize interferences from other heavy metals that might be present in the water sample
    Precautions
    Precautions

    2,4,6- Tris-(2’ - Pyridyl )-s-Triazine ( TPTZ ) or  also can be use like 2,4,6-Tripyridyl-s-triazine. Solution of (TPTZ), .001 M were prepared by dissolving 0.312 gram of compound in a view drops of hydrochloric acid and diluting to 1 ltr with water.

      2,4,6-Tripyridyl-s-triazine solution of (TPTZ) is useful for the calorimetric determination of iron. The Intense violet colour formed by TPTZ with iron(II) is due to the ion {Fe(TPTZ)2}+2, with only two of the three pyridyl group of each TPTZ ligand attached to the Iron atom.   This reagent is  also used for determination of iron in silicates, burnt refractories, and limestone etc